δ ccma Search Results


δ arca  (BioResource International Inc)
90
BioResource International Inc δ arca
Expression of a PiscR-lacZ fusion TorA activity and Moco content in E. coli mutant strains. ( A ) β-galactosidase activities expressed in Miller units were determined for iscR-lacZ fusion in E. coli BW25113 wild-type strain <t>and</t> <t>Δ</t> nac , Δ fur , Δ torI , Δ arcA , <t>Δ</t> <t>ccmA</t> , Δ IscU , Δ fnr , and Δ iscR strains. The strains were cultivated anaerobically in the presence of 20 mM TMAO for 4 h. β-galactosidase activities were normalized to the OD 600 nm values. Standard deviation was calculated from three biological replicates. ( B ) Quantification of relative amounts of Moco in the E. coli BW25113 wild-type strain and the mutant Δ fnr , Δ arcA , Δ fur , and Δ iscU strains. The strains were grown anaerobically in the presence of 20 mM TMAO for 8 h in the LB medium. Total Moco in crude extracts was oxidized overnight with acidic iodine into its fluorescence derivative, FormA. FormA was separated and quantified by its fluorescence monitored at λ ex = 383 nm and λ em = 450 nm. The black, white, and gray bars represent without the isc operon, with iscRSUAhscABfdx iscX , and with iscSUAhscABfdx-iscX , respectively. ( C ) TMAO reductase activity in the E. coli BW25113 wild-type strain and the mutant Δ fnr , Δ arcA , Δ fur , and Δ iscU strains. The strains were cultivated anaerobically in the presence of 20 mM TMAO. TMAO reductase activities (in units) were normalized to the OD 600 nm values. Standard deviation was calculated from three biological replicates. The black, white, and gray bars represent with either the vector only, or with a plasmid carrying the iscRSUAhscABfdx-iscX or iscSUAhscABfdx-iscX genes, respectively.
δ Arca, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%B4+ccma/%CE%B4+arca/pmc10845959-187-23-61
Average 90 stars, based on 1 article reviews
δ arca - by Bioz Stars, 2026-10
90/100 stars
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wild type atcc hg0266 δ ccmf  (ATCC)
95
ATCC wild type atcc hg0266 δ ccmf
Physiological impacts of trx mutations during aerobiosis. (A) Growth of the <t>wild</t> <t>type</t> (WT) and indicated mutants in LB under aerobic conditions. Fresh LB was inoculated with mid-log-phase cultures grown from a single fresh colony to an OD600 of ∼0.01 (∼200× dilution). (B) Plating efficiency of indicated strains on LB plates. Cultures prepared to contain approximately 108 CFU/ml were regarded as the undiluted cultures (dilution factor, 0), which were subjected to 10-fold series dilution. Five microliters of each dilution was dropped on agar plates. Results were recorded after incubation times (IT) of 18 and 30 h. Genetic complementation by expressing a copy of trxA (ptrxA) and trxB (ptrxB) in trans is shown; in Fig. S1C, it is shown for growth in liquid LB. (C) Colors of cell pellets and heme c contents. The heme c levels of the WT (positive control) and ΔccmF strain (negative control) were set as 1 and 0, respectively. The relative content (RC) of each strain after normalization to the WT content is shown. Experiments were performed independently at least 5 times, and data are presented as means ± SEM or as values representative of similar results.
Wild Type Atcc Hg0266 δ Ccmf, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%B4+ccma/Shewanella+oneidensis/pmc06803319-510-73-75
Average 95 stars, based on 1 article reviews
wild type atcc hg0266 δ ccmf - by Bioz Stars, 2026-10
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normal kalilauge  (Anwendung GmbH)
90
Anwendung GmbH normal kalilauge
Physiological impacts of trx mutations during aerobiosis. (A) Growth of the <t>wild</t> <t>type</t> (WT) and indicated mutants in LB under aerobic conditions. Fresh LB was inoculated with mid-log-phase cultures grown from a single fresh colony to an OD600 of ∼0.01 (∼200× dilution). (B) Plating efficiency of indicated strains on LB plates. Cultures prepared to contain approximately 108 CFU/ml were regarded as the undiluted cultures (dilution factor, 0), which were subjected to 10-fold series dilution. Five microliters of each dilution was dropped on agar plates. Results were recorded after incubation times (IT) of 18 and 30 h. Genetic complementation by expressing a copy of trxA (ptrxA) and trxB (ptrxB) in trans is shown; in Fig. S1C, it is shown for growth in liquid LB. (C) Colors of cell pellets and heme c contents. The heme c levels of the WT (positive control) and ΔccmF strain (negative control) were set as 1 and 0, respectively. The relative content (RC) of each strain after normalization to the WT content is shown. Experiments were performed independently at least 5 times, and data are presented as means ± SEM or as values representative of similar results.
Normal Kalilauge, supplied by Anwendung GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%B4+ccma/normal+kalilauge/10__1515_slash_zpch___1901___3834-95-25-24
Average 90 stars, based on 1 article reviews
normal kalilauge - by Bioz Stars, 2026-10
90/100 stars
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Image Search Results


Expression of a PiscR-lacZ fusion TorA activity and Moco content in E. coli mutant strains. ( A ) β-galactosidase activities expressed in Miller units were determined for iscR-lacZ fusion in E. coli BW25113 wild-type strain and Δ nac , Δ fur , Δ torI , Δ arcA , Δ ccmA , Δ IscU , Δ fnr , and Δ iscR strains. The strains were cultivated anaerobically in the presence of 20 mM TMAO for 4 h. β-galactosidase activities were normalized to the OD 600 nm values. Standard deviation was calculated from three biological replicates. ( B ) Quantification of relative amounts of Moco in the E. coli BW25113 wild-type strain and the mutant Δ fnr , Δ arcA , Δ fur , and Δ iscU strains. The strains were grown anaerobically in the presence of 20 mM TMAO for 8 h in the LB medium. Total Moco in crude extracts was oxidized overnight with acidic iodine into its fluorescence derivative, FormA. FormA was separated and quantified by its fluorescence monitored at λ ex = 383 nm and λ em = 450 nm. The black, white, and gray bars represent without the isc operon, with iscRSUAhscABfdx iscX , and with iscSUAhscABfdx-iscX , respectively. ( C ) TMAO reductase activity in the E. coli BW25113 wild-type strain and the mutant Δ fnr , Δ arcA , Δ fur , and Δ iscU strains. The strains were cultivated anaerobically in the presence of 20 mM TMAO. TMAO reductase activities (in units) were normalized to the OD 600 nm values. Standard deviation was calculated from three biological replicates. The black, white, and gray bars represent with either the vector only, or with a plasmid carrying the iscRSUAhscABfdx-iscX or iscSUAhscABfdx-iscX genes, respectively.

Journal: Microbiology Spectrum

Article Title: Iron limitation indirectly reduces the Escherichia coli torCAD operon expression by a reduction of molybdenum cofactor availability

doi: 10.1128/spectrum.03480-23

Figure Lengend Snippet: Expression of a PiscR-lacZ fusion TorA activity and Moco content in E. coli mutant strains. ( A ) β-galactosidase activities expressed in Miller units were determined for iscR-lacZ fusion in E. coli BW25113 wild-type strain and Δ nac , Δ fur , Δ torI , Δ arcA , Δ ccmA , Δ IscU , Δ fnr , and Δ iscR strains. The strains were cultivated anaerobically in the presence of 20 mM TMAO for 4 h. β-galactosidase activities were normalized to the OD 600 nm values. Standard deviation was calculated from three biological replicates. ( B ) Quantification of relative amounts of Moco in the E. coli BW25113 wild-type strain and the mutant Δ fnr , Δ arcA , Δ fur , and Δ iscU strains. The strains were grown anaerobically in the presence of 20 mM TMAO for 8 h in the LB medium. Total Moco in crude extracts was oxidized overnight with acidic iodine into its fluorescence derivative, FormA. FormA was separated and quantified by its fluorescence monitored at λ ex = 383 nm and λ em = 450 nm. The black, white, and gray bars represent without the isc operon, with iscRSUAhscABfdx iscX , and with iscSUAhscABfdx-iscX , respectively. ( C ) TMAO reductase activity in the E. coli BW25113 wild-type strain and the mutant Δ fnr , Δ arcA , Δ fur , and Δ iscU strains. The strains were cultivated anaerobically in the presence of 20 mM TMAO. TMAO reductase activities (in units) were normalized to the OD 600 nm values. Standard deviation was calculated from three biological replicates. The black, white, and gray bars represent with either the vector only, or with a plasmid carrying the iscRSUAhscABfdx-iscX or iscSUAhscABfdx-iscX genes, respectively.

Article Snippet: BW25113 (referred to as parental strain) and the isogenic mutant strains Δ torA, Δ fnr, Δ fur, Δ nac, Δ torI, Δ narL, Δ ccmA, Δ arcA, Δ iscR, Δ modE, Δ moaA, Δ moaC, Δ moaD, Δ moaE, Δ moeA, Δ moeB, Δ mogA, Δ mobA, Δ mocA, and Δ iscU were obtained from the Keio collection from the National BioResource Project (National Institute of Genomics, Japan) ( , ).

Techniques: Expressing, Activity Assay, Mutagenesis, Standard Deviation, Fluorescence, Plasmid Preparation

Physiological impacts of trx mutations during aerobiosis. (A) Growth of the wild type (WT) and indicated mutants in LB under aerobic conditions. Fresh LB was inoculated with mid-log-phase cultures grown from a single fresh colony to an OD600 of ∼0.01 (∼200× dilution). (B) Plating efficiency of indicated strains on LB plates. Cultures prepared to contain approximately 108 CFU/ml were regarded as the undiluted cultures (dilution factor, 0), which were subjected to 10-fold series dilution. Five microliters of each dilution was dropped on agar plates. Results were recorded after incubation times (IT) of 18 and 30 h. Genetic complementation by expressing a copy of trxA (ptrxA) and trxB (ptrxB) in trans is shown; in Fig. S1C, it is shown for growth in liquid LB. (C) Colors of cell pellets and heme c contents. The heme c levels of the WT (positive control) and ΔccmF strain (negative control) were set as 1 and 0, respectively. The relative content (RC) of each strain after normalization to the WT content is shown. Experiments were performed independently at least 5 times, and data are presented as means ± SEM or as values representative of similar results.

Journal: Applied and Environmental Microbiology

Article Title: Distinct Roles of Shewanella oneidensis Thioredoxin in Regulation of Cellular Responses to Hydrogen and Organic Peroxides

doi: 10.1128/AEM.01700-19

Figure Lengend Snippet: Physiological impacts of trx mutations during aerobiosis. (A) Growth of the wild type (WT) and indicated mutants in LB under aerobic conditions. Fresh LB was inoculated with mid-log-phase cultures grown from a single fresh colony to an OD600 of ∼0.01 (∼200× dilution). (B) Plating efficiency of indicated strains on LB plates. Cultures prepared to contain approximately 108 CFU/ml were regarded as the undiluted cultures (dilution factor, 0), which were subjected to 10-fold series dilution. Five microliters of each dilution was dropped on agar plates. Results were recorded after incubation times (IT) of 18 and 30 h. Genetic complementation by expressing a copy of trxA (ptrxA) and trxB (ptrxB) in trans is shown; in Fig. S1C, it is shown for growth in liquid LB. (C) Colors of cell pellets and heme c contents. The heme c levels of the WT (positive control) and ΔccmF strain (negative control) were set as 1 and 0, respectively. The relative content (RC) of each strain after normalization to the WT content is shown. Experiments were performed independently at least 5 times, and data are presented as means ± SEM or as values representative of similar results.

Article Snippet: When necessary, the growth medium was supplemented with chemicals at the following concentrations: 2,6-diaminopimelic acid (DAP), 0.3 mM; kanamycin, 50 μg/ml; gentamicin, 15 μg/ml; and catalase on plates, 2,000 U/ml. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain or plasmid Description Source or reference E. coli strains DH5α Host strain for plasmids Laboratory stock WM3064 Donor strain for conjugation; Δ dapA W. Metcalf, UIUC a S. oneidensis strains MR-1 (ATCC 700550) Wild type ATCC HG0266 Δ ccmF derived from MR-1 23 HG0406 Δ trxA derived from MR-1 This study HG0452 Δ trxC derived from MR-1 This study HG0696 Δ dsbD derived from MR-1 28 HG0976 Δ ohrA derived from MR-1 18 HG0977 Δ ohrR derived from MR-1 19 HG1070 Δ katB derived from MR-1 18 HG1328 Δ oxyR derived from MR-1 18 HG1563 ΔSO_1563 derived from MR-1 19 HG2303 Δ trxB derived from MR-1 This study HG2745 Δ grxA derived from MR-1 This study HG4702 Δ gor derived from MR-1 This study TRXAC Δ trxA Δ trxC derived from MR-1 This study TRXAKATB Δ trxA Δ katB derived from MR-1 This study TRXAOXYR Δ trxA Δ oxyR derived from MR-1 This study TRXAOHRA Δ trxA Δ ohrA derived from MR-1 This study TRXAOHRR Δ trxA Δ ohrR derived from MR-1 This study TRXASO1563 Δ trxA ΔSO_1563 derived from MR-1 This study TRXAPGRXA Δ trxA /P tac :: grxA derived from MR-1 This study Plasmids pHGM01 Ap r Gm r Cm r suicide vector 23 pHGE-P tac IPTG-inducible P tac expression vector 50 pHGEI01 Integrative lacZ reporter vector 36 pBBR-Cre Helper vector for antibiotic marker removal 51 pHGEI01-P katB For measuring P katB activity 20 pHGEI01-P ohrA For measuring P ohrA activity 19 pHGEI01-P trxA For measuring P trxA activity This study pHGEI01-P trxB For measuring P trxB activity This study pHGEI01-P trxC For measuring P trxC activity This study pHGEI01-P gor For measuring P gor activity This study pHGEI01-P grxA For measuring P grxA activity This study pHGE-P tac-trxA Vector for inducible expression of trxA This study pHGE-P tac-trxB Vector for inducible expression of trxB This study pHGE-P tac-trxC Vector for inducible expression of trxC This study pHGE-P tac-grxA Vector for inducible expression of grxA This study pHGE-P tac-dsbD Vector for inducible expression of dsbD 28 pHGE-P tac-ohrR-gfp Vector for inducible expression of ohrR-gfp This study pHGE-P tac-ohrA Vector for inducible expression of ohrA This study Open in a separate window a UIUC, University of Illinois Urbana-Champaign.

Techniques: Incubation, Expressing, Positive Control, Negative Control

Impacts of trx and grx mutations on disulfide bond reduction. (A and B) Growth of the wild type and trxA mutants in LB in the presence of DTT and diamide. Numbers shown are concentrations of chemicals. (C) Susceptibilities of the indicated strains to 2 M diamide as determined by inhibition assays. Experiments were performed independently at least 3 times, and data are presented as means ± SEM or as values representative of similar results.

Journal: Applied and Environmental Microbiology

Article Title: Distinct Roles of Shewanella oneidensis Thioredoxin in Regulation of Cellular Responses to Hydrogen and Organic Peroxides

doi: 10.1128/AEM.01700-19

Figure Lengend Snippet: Impacts of trx and grx mutations on disulfide bond reduction. (A and B) Growth of the wild type and trxA mutants in LB in the presence of DTT and diamide. Numbers shown are concentrations of chemicals. (C) Susceptibilities of the indicated strains to 2 M diamide as determined by inhibition assays. Experiments were performed independently at least 3 times, and data are presented as means ± SEM or as values representative of similar results.

Article Snippet: When necessary, the growth medium was supplemented with chemicals at the following concentrations: 2,6-diaminopimelic acid (DAP), 0.3 mM; kanamycin, 50 μg/ml; gentamicin, 15 μg/ml; and catalase on plates, 2,000 U/ml. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain or plasmid Description Source or reference E. coli strains DH5α Host strain for plasmids Laboratory stock WM3064 Donor strain for conjugation; Δ dapA W. Metcalf, UIUC a S. oneidensis strains MR-1 (ATCC 700550) Wild type ATCC HG0266 Δ ccmF derived from MR-1 23 HG0406 Δ trxA derived from MR-1 This study HG0452 Δ trxC derived from MR-1 This study HG0696 Δ dsbD derived from MR-1 28 HG0976 Δ ohrA derived from MR-1 18 HG0977 Δ ohrR derived from MR-1 19 HG1070 Δ katB derived from MR-1 18 HG1328 Δ oxyR derived from MR-1 18 HG1563 ΔSO_1563 derived from MR-1 19 HG2303 Δ trxB derived from MR-1 This study HG2745 Δ grxA derived from MR-1 This study HG4702 Δ gor derived from MR-1 This study TRXAC Δ trxA Δ trxC derived from MR-1 This study TRXAKATB Δ trxA Δ katB derived from MR-1 This study TRXAOXYR Δ trxA Δ oxyR derived from MR-1 This study TRXAOHRA Δ trxA Δ ohrA derived from MR-1 This study TRXAOHRR Δ trxA Δ ohrR derived from MR-1 This study TRXASO1563 Δ trxA ΔSO_1563 derived from MR-1 This study TRXAPGRXA Δ trxA /P tac :: grxA derived from MR-1 This study Plasmids pHGM01 Ap r Gm r Cm r suicide vector 23 pHGE-P tac IPTG-inducible P tac expression vector 50 pHGEI01 Integrative lacZ reporter vector 36 pBBR-Cre Helper vector for antibiotic marker removal 51 pHGEI01-P katB For measuring P katB activity 20 pHGEI01-P ohrA For measuring P ohrA activity 19 pHGEI01-P trxA For measuring P trxA activity This study pHGEI01-P trxB For measuring P trxB activity This study pHGEI01-P trxC For measuring P trxC activity This study pHGEI01-P gor For measuring P gor activity This study pHGEI01-P grxA For measuring P grxA activity This study pHGE-P tac-trxA Vector for inducible expression of trxA This study pHGE-P tac-trxB Vector for inducible expression of trxB This study pHGE-P tac-trxC Vector for inducible expression of trxC This study pHGE-P tac-grxA Vector for inducible expression of grxA This study pHGE-P tac-dsbD Vector for inducible expression of dsbD 28 pHGE-P tac-ohrR-gfp Vector for inducible expression of ohrR-gfp This study pHGE-P tac-ohrA Vector for inducible expression of ohrA This study Open in a separate window a UIUC, University of Illinois Urbana-Champaign.

Techniques: Inhibition

Strains and plasmids used in this study

Journal: Applied and Environmental Microbiology

Article Title: Distinct Roles of Shewanella oneidensis Thioredoxin in Regulation of Cellular Responses to Hydrogen and Organic Peroxides

doi: 10.1128/AEM.01700-19

Figure Lengend Snippet: Strains and plasmids used in this study

Article Snippet: When necessary, the growth medium was supplemented with chemicals at the following concentrations: 2,6-diaminopimelic acid (DAP), 0.3 mM; kanamycin, 50 μg/ml; gentamicin, 15 μg/ml; and catalase on plates, 2,000 U/ml. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain or plasmid Description Source or reference E. coli strains DH5α Host strain for plasmids Laboratory stock WM3064 Donor strain for conjugation; Δ dapA W. Metcalf, UIUC a S. oneidensis strains MR-1 (ATCC 700550) Wild type ATCC HG0266 Δ ccmF derived from MR-1 23 HG0406 Δ trxA derived from MR-1 This study HG0452 Δ trxC derived from MR-1 This study HG0696 Δ dsbD derived from MR-1 28 HG0976 Δ ohrA derived from MR-1 18 HG0977 Δ ohrR derived from MR-1 19 HG1070 Δ katB derived from MR-1 18 HG1328 Δ oxyR derived from MR-1 18 HG1563 ΔSO_1563 derived from MR-1 19 HG2303 Δ trxB derived from MR-1 This study HG2745 Δ grxA derived from MR-1 This study HG4702 Δ gor derived from MR-1 This study TRXAC Δ trxA Δ trxC derived from MR-1 This study TRXAKATB Δ trxA Δ katB derived from MR-1 This study TRXAOXYR Δ trxA Δ oxyR derived from MR-1 This study TRXAOHRA Δ trxA Δ ohrA derived from MR-1 This study TRXAOHRR Δ trxA Δ ohrR derived from MR-1 This study TRXASO1563 Δ trxA ΔSO_1563 derived from MR-1 This study TRXAPGRXA Δ trxA /P tac :: grxA derived from MR-1 This study Plasmids pHGM01 Ap r Gm r Cm r suicide vector 23 pHGE-P tac IPTG-inducible P tac expression vector 50 pHGEI01 Integrative lacZ reporter vector 36 pBBR-Cre Helper vector for antibiotic marker removal 51 pHGEI01-P katB For measuring P katB activity 20 pHGEI01-P ohrA For measuring P ohrA activity 19 pHGEI01-P trxA For measuring P trxA activity This study pHGEI01-P trxB For measuring P trxB activity This study pHGEI01-P trxC For measuring P trxC activity This study pHGEI01-P gor For measuring P gor activity This study pHGEI01-P grxA For measuring P grxA activity This study pHGE-P tac-trxA Vector for inducible expression of trxA This study pHGE-P tac-trxB Vector for inducible expression of trxB This study pHGE-P tac-trxC Vector for inducible expression of trxC This study pHGE-P tac-grxA Vector for inducible expression of grxA This study pHGE-P tac-dsbD Vector for inducible expression of dsbD 28 pHGE-P tac-ohrR-gfp Vector for inducible expression of ohrR-gfp This study pHGE-P tac-ohrA Vector for inducible expression of ohrA This study Open in a separate window a UIUC, University of Illinois Urbana-Champaign.

Techniques: Plasmid Preparation, Conjugation Assay, Derivative Assay, Expressing, Marker, Activity Assay